The super-resolution microscope is a new generation of microscope known as a nanoscope. STED (stimulated-emission-depletion) is one of a different super-resolution microscopy techniques that can be used together with the following:
- dynamic super-resolution technique…
Principle of the STED
In the STED (stimulated-emission-depletion) technique, part of the sample is activated by a laser and the periphery of the excited area is then switched off by stimulated emission (de-excitation of an electron). Only the centre of the excited area remains fluorescent. The use of scanning in this technique produces a set of points and therefore forms a large accurate image. Resolution is increased as far as 35 nanometres.
The principle of STED microscopy. On the left: transverse view of an excitatory beam. In the centre: de-excitatory beam. On the right: resulting fluorescence. © Lexic_4712, Wikipedia commons by-sa 3.0