The light field microscope unlike the dark background microscope collects light from the source directly in the lens after it has passed through the sample. This is lit from beneath and observed from above. Although sample preparation is uncomplicated, the technique is limited by a low contrast and poor resolution. Observation can be improved by staining the samples.
This microscope can be used to visualise various living (bacteria, unicellular organisms, etc.) and fixed (histological sections, etc.) objects.
Picture of an aphid under clear field microscopy. © Pest and Diseases Image Library, bugwood.org, CC by-nc 3.0